ABSTRACT
Finger millet Eleusine coracana L., commonly called Ragi, is a rich source of phytochemicals and have number of health beneficial effects. The present study evaluated the total antioxidant activity (TAA), condensed tannins (CT), micronutrient content (Fe & Zn), diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and phenolic compounds in 35 finger millet genotypes. The assayed genotypes showed 0.91-0.99 mg/g CT, 23.79-56.51 mM/kg TAA, 1.76-44.47 µM/g DPPH scavenging activity, 44.14-88.09 µg/mL ABTS activity, 100-463.53 µM FRAP value, 37.04-69.13 ppm Fe and 28.94-46.77 ppm Zn. HPLC analysis showed that gallic, tannic, ferulic, caffeic and o-coumaric acid to be major polyphenols in all genotypes. Principal component analysis (PCA) revealed significantly higher CT, TAA with relatively good amount of Fe and Zn in VL Ragi 146, VL Mandua 352, VL 336, VL 373, VL 325, VL 351, GPHCPB 7, GPHCPB 3, GPHCPB 52 and VR 708 genotypes. Agglomerative hierarchical cluster analysis classified the 35 genotypes into two clusters; Cluster I had higher CT, TAA, FRAP, DPPH, ABTS, while cluster II recorded higher Fe and Zn. This study clearly demonstrated the nutraceutical properties with higher antioxidant potential of identified genotypes, which can be suitably deployed for nutritional security, particularly in developing countries.
ABSTRACT
Objective:To determine quantitative phytochemical, anticancer and antidiabetic effect of seven Indian tropical fruit residues. Methods:In-vitro cytotoxic activity (IC50) was evaluated against cervical cancer cells (HeLa), breast cancer cells (MCF-7), hepatocellular carcinoma cells (HepG-2) and bone sarcoma cells (MG-63) and alpha amylase inhibition assay was used for antidiabetic activity. Results: Results of phytochemical analysis revealed that all residues contained remarkable amount of alkaloid, saponin, tannin and flavonoid. Notable cancer cell growth inhibition was observed for the extract from Carissa carandas pomace and Litchi sinensis seeds with IC50 values ranged from 56.72 to 89.24 μg/mL. Alpha amylase inhibition assay was measured at six different concentrations (5, 10, 25, 50, 100 and 200 mg/mL) by using different solvent extract. Results showed that Carissa carandas possessed best activity with IC50 value as 29.66 mg/mL followed by other residues in methanol extract. Conclusions:Study suggests that these fruit residues demonstrate promising antidiabetic and anticancer activity that substantiated its ethno medicinal use and may provide new molecules for the treatment of these diseases.
ABSTRACT
Genomic DNA was extracted from eight medicinal plants using the present DNA extraction protocols (CTAB extraction method) with some modifications. Leaves were fixed in different fixing solutions containing absolute alcohol (99.99%), chloroform and EDTA, but without liquid nitrogen. DNA quality and quantity obtained were comparable to those isolated with liquid nitrogen, as the λ260/λ280 ratio with liquid nitrogen was in range 1.3-1.7 and with other fixing solutions it was 1.1-1.5. Absolute alcohol showed best results as fixing solution. Good quality of DNA was isolated without using liquid nitrogen from different medicinal plant species. DNA isolated by this method was suitable for various molecular biology applications.